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Fill in the blanks to compare bacterial vs eukaryotic TRANSLATION. Instead of a

ID: 218424 • Letter: F

Question

Fill in the blanks to compare bacterial vs eukaryotic TRANSLATION.

Instead of a Shine-Dalgarno sequence, eukaryotic mRNA's use a structure called ___ to bind with the ___ subunit of ribosomes. Therefore the eukaryotic cells need initiation factor ___ whereas bacterial cells do not. In eukaryotes, but not bacteria, the mRNA sequence needed for optimal translation initiation follows ____

Bacteria require ___ , which is located in the ____ site of the large subunit, to join amino acids together by forming ___ bonds.

Bacteria and eukaryotes both have proteins called ____ that mimic the shape of a tRNA; these proteins bind to the ___ and when they do, translation is ___ .

Explanation / Answer

Instead of a Shine-Dalgarno sequence, eukaryotic mRNA's use a structure called Kozak consenus sequence (G/ANNAUGG) to bind with the smaller (40s) subunit of ribosomes. therefore the eukaryotic cells need initiation factor 4G whwereas bacterial cells do not .in eukaryotes, but not bacteria , the mRNA sequence needed for optimal translation initiation follows 5' CAP .

Bacteria require EF-G/Tu (elongation factor -G) , ehich is located in the A -site of the large subunit , to join amino acids together by forming Peptide bonds.

Bacteria and eukaryotes both have proteins Release Factors 1,2 (RF 1,2) that mimic the shape of a t-RNA ; these proteins bind to the stop codon and when they do , translation is Terminated.

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