af engineering of bacteria is often accomplishod through transformation Successt

Question

af engineering of bacteria is often accomplishod through transformation Successtul trancformation the engineered DNA to be brought into the microbe. What bucteria depends on them having proteins allowing t is the name for these proteins? a) DNA polymerases b) Ligases c) Competence factors d) F plasmid proteins 15. Which of the following statements are true reasons that genetic engineering is so a) Microbes b) Genetically engineered microbes can produce some valuable compounds while requiring can create complex biological molecules that are difficult or expensive to produce otherwise fewer toxic chemicals or industrial processes than previous production methods did can be engineered to produce protein using human genetic information, allowing humans to be treated with human proteins instead of those harvested from other animals d) A and B, but not C e) A, B, and C 16. During the logarithmic ("log") phase of microbial population growth, metabolic processes associated with growth generally produce small byproduct molecules known as a) glycolysis b) primary metabolites c) secondary metabolites d) tertiary metabolites 17. During the phase of microbial population growth, nutrients become scarce and population levels reach a plateau. Microbes produce larger, more complex metabolites often associated with processes like lag/competition and survival a) b) lag/DNA replication c) stationary /competition and survival d) stationary/DNA replication 18. Imagine that you want to make a plant that produces a protein that makes the plant taste bad to insects. We would typically achieve this by: a) Putting the gene for the protein in the Ti plasmid, putting the plasmid in a bacterium, and infecting the b) c) d) plant with the bacterium Changing the plant's chromosome directly by cutting it and adding the gene for the protein we want Putting the gene for the protein of interest in a bacteriophage and infecting the plant with it Enriching the soil with many copies of the protein we want the plant to produce 19. Things we can accomplish using genetic engineering include: a) Engineering bacteria to produce human proteins that we can harvest for human use b) Engineering plants to be drought resistant c) Engineering plants to be more nutritious d) All of the above 20. Crown gall disease, which affects plants, is caused by a) Infection by a virus whose genetic material encodes for tumor-causing proteins b) Infection by a bacterium whose plasmid integrates with the plant chromosome c) Infection by a fungus which stores its spores within the plant tissue d) Spontaneous genetic mutations causing tumors Page 4

Explanation / Answer

14. (c) competence factor

15. (e) A B & C

16. (c) Secondary metabolites

17. (c) stationary phase/ competetion and survival

18. (c) putting the gene for the protein of interest in a bacteriophage and infecting the plant with it.

19. (d) All of the above

20. (b) Infection by bacterium whose plasmid integrates with the plant chromosome

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