PART 3: CREATE YOUR OWN CIRCUIT By the end of the quarter we would like you to b
ID: 210777 • Letter: P
Question
PART 3: CREATE YOUR OWN CIRCUIT By the end of the quarter we would like you to be comfortable with regulating a gene or set of genes using positive and/or negative regulation. We have provided a gene below that needs to be regulated given the infor- mation in the different scenarios. Your goal is to use transcription factors and small molecules to regulate the expression of the gene at the transcriptional level. Regulatory Region GUA Promoter Region Coding Region The gene GUA7 above encodes for an enzyme that catalyzes the production of the nitrog- enous base Guanine. 1. Guanine is used as a building block for what macromolecule? Nucher Aeid 2. When levels of Guanine are owin the cell. should the cell INCREASE or DECREASE (circle) the expression of GUExplanation / Answer
1. Guanine is used to make nucleic acid
2. If the operon is positively regulated then low guanine levels would inactivate operon, decrease the expression
If the operon is negatively regulates then low guanine levels would activate the operon this increase the expression
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