4. Commercial biochemical test kits used to identify N. gonorrhoeae require an i

Question

4. Commercial biochemical test kits used to identify N. gonorrhoeae require an inoculation fluid Because we are very thrifty in the Microbiology Department, we sometimes make the inoculation fluid instead of buying it 'ready-made'. The recipe for making the inoculation fluid is as follows: KCI 6.0 g CaCl2 0.5 g .0.1 N NaOH 1.6 ml deionized H2O to 1000 ml Assume you want to make exactly 225 mls of the inoculation fluid. (Refer to See Handout 5.2- International Atomic Masses and Handout 5.3- Molarity and Normality to answer the following questions.) . You will add grams of KCl to 225 mls of H2O. . You will add grams] of CaCl2 to 225 mls of H.O . You will add milligrams of KCL and milligrams of CaCl2 to 225 mls of H20 . You have 500 mls of a 10M NaOH stock solution. You want to make 50 mls of 0.1 N NaOH. You will dilute mis 10M NaOH in 50 mls of H2O.

Explanation / Answer

In this question, calculate for the 225 ml concentrations of different components

For 1000 ml 6g of KCl is required.

For 1 ml is required is 6/1000

For 225 ml need = 6* 225/1000= 1.35 gm is required

Similarly CaCl2

1000 ml is required for the 0.5gm

For 225 ml 0.5*225/1000=0.1125 gm is required or 112.5 mg is required

112.5 mg of CaCl2

Here is 10 N of stock solution

10 * xml= 0.1* 50

X= 5/10

X= 0.5 ml of 10 N NaOH solution is required to make 50 ml of 0.1 N NaoH solution. In this remember one thing that final volume is 50 ml

Related Questions

Navigate

• Browse (All)
• Browse 4
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.