In salting out of protein experiment which fraction give positive results with b

Question

In salting out of protein experiment which fraction give positive results with biuret reagent? Explain your answer? 6- YOUR RESULTS Filtrate Residue on filter paper Color change Positive or negative result What is the strongest possible interaction between the side chains of each of the following pairs of amino acids? Choose from covalent bonds, van der Waals forces, ionic bonds, and hydrogen bonds? 7- Strongest Interaction Amino Acids Ser and Gln Ala and Met Your enzyme oxidizes compound W and in the process produces a faint yellow glow, so assaying activity of your enzyme is easy. You are ready to purify your enzyme. You begin with a crude lysate of bacteria, and after centrifugation, you find activity only in the supernatant. You first precipitate proteins out of the crude lysate supernatant at 15% ammonium sulfate, but there was no activity in the 15% ammonium sulfate pellet. You increase the amount of ammonium sulfate in the supernatant from 15% to 25% but there was no activity in the 25% ammonium sulfate pellet. You increase the amount ofammonium sulfate in the supernatant from 25% to 45%, and now all of the activity is in the pellet. In the lab, you precipitated -galactosidase out of the crude lysate supernatant at 45% ammonium sulfate. Compare the precipitation above for enzyme X and the one in the lab for p-gal. Was one a better purification than the other or were they the same? Explain your answer 8.

Explanation / Answer

6)

In biuret test Copper(II) forms violet colored coordination complexes in alkaline solution affirming the presence of peptide bonds. Since in salting out, the proteins salt out, i.e become insoluble and precipitate out, the residue will give a positive result, affirmed by a violet color, the filtrate will remain to be light blue colored upon addition of reagent

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