A. Protein-X has about 200 kDa of molecular weight B. Gene-X may generate multip

Question

A. Protein-X has about 200 kDa of molecular weight

B. Gene-X may generate multiple gene products

C. Antibody-A and -B recognizes the same proteins

D. Antibody-A is polyclonal

E. Antibody-B is polyclonal

To study the function of gene-X, you generated a Knockout of gene-X. You obtained two antibodies against a possible gene product of gene-X, Protein-X from the two different vendors and performed Western blotting on the total lysates from wild-type and from the Knockout. Which of the following statements is correct? Wild-type Knockout-X Wild-type Knockout-X 200kDa 100kDa _100kDa 50kDa 50kDa 35kDa 35kDa Antibody-A Antibody-B

Explanation / Answer

Gene x would be producing multiple genes. This is because the 2 antibodies give different and multiple bands.

Not (a) because in that case there should have been no band in case of knockout if the gene.

Not ,(c) you get different sets of bands in both the antibodies.

None can be claimed to be polygonal given this data.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.