A. Provide the function for the following enzymes. Topoisomerase Helicase Primas

Question

A. Provide the function for the following enzymes.

Topoisomerase

Helicase

Primase

DNA Polymerase I

DNA Polymerase III

Ligase

B. The study of changes in gene expression that occurs without alteration to genes is called

_________.

C. Noncoding regions in eukaryotic DNA are called ________. These have _______ functions

and alter gene expression.

D. Shift pairing of RNA at the third position is called ____________.

E. An operon is composed of _____.

F. In the Lac operon, if lactose is present, the operator of the lac operon is switched ____;

therefore, this is an example of a(n) ______ operon.

G. Name the 4 methods of horizontal gene transmission and how each occurs.

Explanation / Answer

Helicase : It is often used to seperate strand of DNA double helix or self anneal RNA molecules by using energy from ATP.

Topoisomerase: Topoisomerases catalyze and guide the unknotting or unlinking of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.

Primase : In bacteria Primase bind to DNA helicase forming a complax call prisome.

DNA polymerase I : It is an enzyme that participate in the process of prokaryotic DNA replication .

DNA polymerase II :It is prokaryotic DNA dependent DNA polymerase.DNA Pol II is not the most studied polymerase so there are many proposed functions of this enzyme which are all likely functions but are ultimately unconfirmed

Ligase : DNA ligase is specific type of enzyme that facililate the jioning of DNA strand together by catalyzing formation phosphodiaester bond . it is play role in repairing singal strand break into duplex DNA.

B) epigenetics

C) Introns

D) Wobble base pair

E) promotore , operator , stuctural genes.

F)

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