Figure 1 Expression of miR 203 during kentinocye dftereniaon (a) Primary mouse k

ID: 192580 • Letter: F

Question

Figure 1 Expression of miR 203 during kentinocye dftereniaon (a) Primary mouse keratinocypes were solated as dishes. Belore they seached condiuence,cels were induced to diferentate by adding 1 2 md Cacl, to the cuiture medium perform nnem blot ,nd western bet analysts Hybriduaton to detect U2snRNA was used as a oading oontrol milian densitometry and seuts are reported as fold chenge over zero ine Fiesults oblta ned from the densilometry are shown as Differentiation was evaluated by westem blot for inwolucrin,&Np63; protein downnegulation during dterentiation is also shown actin i show the quentitative laser denstometty of mA 203 (fold over controk black bers) and p63 espression es of reduction; white bars) as mean experiments. (bj Human epidermal keratinocyles (HEKs) were outused in KBM medium with KGM-2 growth supplements Cells wre CaCl, to the cuiture medium Cells were coilected at the indicaled Sme points to perform northem bot and westen blo a koading contro. MR 203 expression wes normakzes to U2s FINA by densitometry, and resuits are neported as fold change over zeo time. oferents desoribed in Malerials meen ± SD torn three indep used as loading cor ± SD 15or induced to dilenertie dersomory of miR203 Oold over contr t black bars) and p63 expressen 0% of reduton whee bars) as nan t SDfemmea toorden eepe nt 1 3 5 days dir m R 203 1 2 3 4 anos 1 2 34lane days dinm (KDa) 3 5 7days di KDa) 135 ANp63 48 48 acin 1 2 3 3 4lanes 1 23 4 nes 12 120 mR.203 16 80 40 0 1 3 5 days diff. 013 5 days diff. A northern blot was performed to detect miR-203 in the top panels of Figure 1a and b. What kind of molecule could be a "probe" in a northern blot? Choose two answers e RNA . DNA e protein . antibody What kind of molecule is the target of the probe in a northern blot? . RNA DNA e protein antibody

Explanation / Answer

Northern blot is a technique used to study gene expression by detection of RNA in a sample.

a>.DNA

.RNA

A nucleotide sequence is used as a probe that can be DNA or RNA or oligonucleotide either labelled with radioactive isotopes or chemilumenscence compound which has sequence complementarity to the RNA of interest.

b).RNA

The target of the probe will be RNA of interest.

Navigate

Figure 1 E4 E2 3. Refer to Figure 1 above. Which of the following shifts show th

Figure 1 Figure 1 - Series RC Circuit Table 1 - RC Circuit Calculated Impedance

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.

Figure 1 Expression of miR 203 during kentinocye dftereniaon (a) Primary mouse k

Question

Explanation / Answer

Related Questions

Navigate