Explain why the movement of myosin along an actin fi lament is \"hopping,\" wher
ID: 190409 • Letter: E
Question
Explain why the movement of myosin along an actin fi lament is "hopping," whereas the movement of kinesin along a microtubule "Hard" keratins such as those found in hair, horn, and nails have a high sulfur content, whereas "soft" keratins found in the skin have a lower sulfur content. Explain the structural basis for this observation. Phalloidin is used as an imaging tool by covalently attaching a fluorescent tag to it. What structures can be visualized in cells treated with fluorescently labeled phalloidin? What is not visible? Myoglobins from three species of fish-tuna, bonito, and mackerel-were purified, and their oxygen binding affinities were measured at 208C. The results are shown in the figure above right. (a) Use the graph to estimate the p50 values for each species. (b) Which species of fish has the highest oxygen-binding affinity at the experimental temperature? The least?Explanation / Answer
1.
2. Phalloidin can be used to visualize and quantitate F-actin in tissue sections or cell cultures.
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