Explain why the absorption spectrum of a molecule is independent of the excitati

Question

Explain why the absorption spectrum of a molecule is independent of the excitation intensity Explain why the emission spectrum of a molecule is independent of the excitation wavelength 3 How do your answers to 1 &2 play out in the working of a fluorescence microscope Lookup DNA, gene, transcription, FISH, & codon on Wikipedia (our reference book for these topics. With FiSH imaging, you can choose to label either an intron or an exon of a gene. What difference does it make? Lookup DAPI& Hoechst on Wikipedia. Is one preferable to the other? 6. 5 Lookup the Molecular Expressions website for basics of the fluorescence microscope (our reference book for this topic, and all of microscopy)

Explanation / Answer

Answer:
1.

Absorption spectrum is define to the part of the absorbed incident radiation by molecules over various different frequencies.
It is the function of composition of material (atomic and molecular). Frequency also depends on many factors like pressure, temperature and electromagnetic field.
Since frequency is independent of intensity. That is why absorption spectrum also not depends on excitation intensity.  

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