Figure 1. Shows the fraction of strains with particular phenotypes during serial

Question

Figure 1. Shows the fraction of strains with particular phenotypes during serial passage into new media. Rate the virulence factors type 1 fimbriae, P fimbraie, and aerobactin as constitutive or inducible, and explain your interpretation.

P values were calculated by using the chi-square statistic or Pisher s exa e Total DNA positive but plasmid-DNA negative with aerobactin Median number of antimicrobial agents (of 12 tested) lo which the strain 100 -T JS 80 60 40 20 7 14 21 Days of Serial Passage (Agar and Broth FIG. 1. Percentage of strains expressing virulence factors when tested repeatedly during serial passage. The results shown are from passage on 5% sheep blood agar (P fimbriae and production on supplemented M9 agar plus 2,2'-dipyridyl (aerobactin brain heart [O), in Luria broth (aerobactin utilization (AD, or in the day(s) infusion broth (type 1 fimbriae [.D. The symbols indicate on which virulence factor tests were performed.

Explanation / Answer

30% percent of strains express virulance factor on 0 day. The pvirulance factor in strains rapidly increased to more than 75% on 7th day. From 7th day to 14th day the percentage of cells expressing virulance factor increased very slowly and reached to 82-84% by 14th day. From 14th day to 21st day there is no increase and the precentage remained almost constant thereafter.

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