Figure 1 Question Question contd: and binding site methylation specifically with

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Figure 1 Question Question contd:
and binding site methylation specifically within acute promydocytic leukaemia cells (NB), andboth interact with promyelokyticleukaemia (PML bodies" a sub nudear structure disrupted in PML cells. The anomalous behaviour ofthese two transcription factors with respect to chromatin structure and DNA methylation may thus be related to a specialized mechanism seen only in pathologically altered cells. A map of distal DHS-to-promoter connections From examination of DNasel profiles across many cell types observed that many known cell-selective enhancers become DHSs nchronously with the appearance of hypersensitivity at the pro- moter of their targetgene (supplementary Fig 13. To generalize this, we analysed the patterning of1A54,901 distal DHSS (DHSs separated from a Tss by at least one other DHS across 79 diverse cell types Distal DHSs conneenad Promoter DHSe connected (Supplementary Methods and Sapplementary Table 6), and corre- per promoter DHS per distal DHS lated the cross-cell type DNase signal at each DHS position with that at all promoters within 500 kb (Supplementary Rig, 14a). We 1-10 identified total of578905DHSethat were highly correlated (r 07 with at least one promoter (P

Explanation / Answer

DNase I cleavage sites indicate that these are in open state i.e. in unwound state free from nucleosomes. This marks the region of active gene expression. While other regions, where they are resistant to DNase I treatment indicate that they in a closed state which states that they are inaccessible for the gene expression.

Not all the genes are expressed in the cell at all times, genes are expressed in a cell type-specific manner. The DNase I cleavage sites appearing in some cell types, means those are expressed in those cell types but not in others.

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