A scientist inserts a DNA fragment encoding a mouse gene into the plasmid shown

Question

A scientist inserts a DNA fragment encoding a mouse gene into the plasmid shown below. The mouse DNA insert is cut with the enzyme XbaI at one end and with EcoRI at the other end. The plasmid is digested with the same two enzymes and combined with the insert in the presence of DNA ligase. The bacteria do not contain a lacZ gene or antibiotic resistance genes of their own.

kan = kanamycin (an antibiotic)

zeo = zeocin (an antibiotic)

R = resistance

Ori = replication origin

Which of the following would be required to promote the high-level expression of the mouse gene inside of bacteria?

A. A bacterial promoter

B. A mouse promoter

C. A and B

Explanation / Answer

To express mouse gene in bacteria, bacterial promoter is required. This is because, bacterial promoter controls the gene expression in bacteria. Eukaryotic (mouse) promoter may not be expressed efficiently in bacteria to produce required high level expression of the transgene. Therefore, option A is correct.

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