A scientist inserts a DNA fragment encoding a mouse gene into the plasmid shown

Question

A scientist inserts a DNA fragment encoding a mouse gene into the plasmid shown below. The mouse DNA insert is cut with the enzyme XbaI at one end and with EcoRI at the other end. The plasmid is digested with the same two enzymes and combined with the insert in the presence of DNA ligase. The bacteria do not contain a lacZ gene or antibiotic resistance genes of their own.

kan = kanamycin (an antibiotic)

zeo = zeocin (an antibiotic)

R = resistance

Ori = replication origin

Which of the following would distinguish bacteria transformed with recombinant plasmid (plasmid containing the mouse gene insert) from those transformed with non-recombinant, uncut plasmid that does not contain the insert?

A. Sensitivity to kanamycin

B. Sensitivity to zeocin

C. Ability to break down X-gal

D. A and B

E. A and C

F. B and C

G. A, B, and C

Explanation / Answer

Which of the following would distinguish bacteria transformed with recombinant plasmid (plasmid containing the mouse gene insert) from those transformed with non-recombinant, uncut plasmid that does not contain the insert?

A. Sensitivity to kanamycin

B. Sensitivity to zeocin

C. Ability to break down X-gal

D. A and B-

E. A and C

F. B and C

G. A, B, and C-----------answer

=============

Transformation is the procedure by which bacterial cells take up the hereditary material other than their own. In the event that the remote DNA has a starting point of replication perceived by the host cell, the microbes will duplicate the outside DNA alongside their own particular DNA.This is finished by a system called "warm stun," which expands the penetrability of the phone film to DNA. The new hereditary material is incorporatedinto the bacterial plasmid. Part of the new "hereditary building" technology.Can be utilized to present valuable new qualities. Plants and creatures can be made impervious to specific maladies by this process.Antibiotic resistance: E. colicannot ordinarily develop within the sight of ampicillin or separate X-lady. The DNA plasmid was put into the microbes. The plasmid has two qualities that code for imperviousness to ampicillin and for a chemical that can separate the X-lady. After change, the microorganisms ought to take in the new DNA, which will be distinguished by their capacity to develop with ampicillin furthermore by their capacity to breakdown X-lady.

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