1. What do expect to see if the bacteria are inhibited by Streptomyces? A. growt
ID: 162731 • Letter: 1
Question
1. What do expect to see if the bacteria are inhibited by Streptomyces?
A. growth along the inoculation line and up to the growth of Streptomyces
B. only growth along the inoculation line at the growth of Streptomyces
C. no growth along the inoculation line where the antibiotic is at high enough concentration to inhibit growth
2. In what direction will you inoculate Staphylococcus epidermidis onto the Penicillium preinoculated plate?
A. from top to bottom staying away from the existing growth of Penicillium
B. from right to left across the plate up to the existing growth of Penicillium
C. from left to right dragging the loop from the Penicillium growth across the plate
3. The ability of Penicillium mold to kill Gram-positive bacteria is an example of
A. Antimicrobial
B. Antibiosis
C. Antibiotic
D. Antiseptic
4. A clear area in which there is no bacterial growth around an antibiotic is known as a zone of inhbition.
A. True
B. False
Explanation / Answer
Please find the answers below:
Answer 1: Choice C (Antibiotics are used to kill bacteria and other microbes both in vitro and in vivo. Normally in microbiological assays, a growth plate is prepared and bacteria are allowed to grow. After that, the target antibiotic is applied and the pattern of bacterial death is noted. This is done by a simple double diffusion assay system. The bacteria residing near the antibiotic wells die more and those residing far away from the antibiotic wells survive more.)
Answer 2: Choice A (When two types of bacterial colonies are required to be grown together, it is pre-requisite to isolate spacious colonies of each type in the culture plate. Thus, if the previous culturing has been streaked from left to right, it is always advised to do next streaking from right to left direction. Since a normal practise involves first streaking from top to right, it will be wise to perform next streaking away from the previous one in top to bottom direction)
Answer 3: Choice C (The scientific term for the agents which kill microbes by impairing their physiological mechanism such as interfering transcription, DNA replication, translation, cellular rupturing by cell wall degradation etc. are termed as antibiotics. These are also routinely used in microbiological experiments)
Answer 4: True (When an antibiotic is applied to a microbiological growth plate, a specific area of bacterial colonies die residing close to the antibiotic well in the gel. This generates a clear zone nearby the antibiotic well and is called zone of inhibition. The radius of this zone is a direct mathematical indicative of extent of activity of the antibiotic.)
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