1. Below you will find a series of events that take place during apoptosis. Deve

Question

1. Below you will find a series of events that take place during apoptosis. Develop a hypothesis about the series of events after the induction of apoptosis. Give the evidence that leads you to hypothesize as such. To indicate the order, you can simply indicate the letter for the appropriate event. Place the events in their proper order (from first to last). New order of events Events A. DNA fragmentation B. Increase in Caspase 3 activity C. Chromatic Condensation D. PARP cleavage E. Cytochrome Crelease

Explanation / Answer

Cellular fractionation is used to measure cytochrome c release. Increase in caspase 3 activity is measured by increase in Fluorescence activity. For measuring PARP activity, the incorporation of radioactivity from isotope-labeled NAD+ into TCA-precipitable proteins is considered the most appropriate. Measuring the distribution of nucleoplasmic proteins in the appropriate size range gives the idea of chromatin condensation. Fragmented DNA can be seen when run on agarose gel.

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