1. Before you start looking at the SBA throat plates that have incubated since y
ID: 148642 • Letter: 1
Question
1. Before you start looking at the SBA throat plates that have incubated since yesterday, the supervisor explains that all the patients first had a positive result on a rapid antigen detecting test kit. A pharyngeal specimen was then taken and plated.
a. Explain what you are looking for on the SBA throat plates.
b. Explain why all the plates are from children.
2. The supervisor explains that they rarely see a culture for otitis media, because the predominant pathogens are known. The doc will just start therapy. The supervisor wants to make sure you know what to do if a specimen for otitis media does come in. Please list the possible pathogenic organisms and explain what media you would require for their growth.
3. You are now asked to read gram stains from sputum samples. Before starting, the supervisor wants to make sure you know the guidelines to follow. Please list the guideline for reading sputum gram stains and explain the reason for each guideline.
4. It is flu season and the micro lab is getting ready. You are helping the supervisor unpack supplies. As you put vials of X and V discs on the shelf, the supervisor asks you to explain what organisms the X and V discs are used for and what is the complete explanation of the principle behind the test?
5. Back at the wound bench, you read a gram stain that has a mixture of gram-positive and gram-negative bacteria. This leads you to believe that it is myonecrosis.
a. You plate the specimen to SBA, CHOC and MAC. What additional plates should you use?
b. What organisms (genus only) are you looking for to grow?
6. You look at the plates from 10 correctly plated fecal samples. You determine that all growth is normal flora and there are no pathogenic organisms growing on any plate. The supervisor agrees with your plate reading, but asks how normal flora of the intestine can affect pathogenic organisms?
7. You are reading plates from a fecal sample and there is growth on the TCBS plate.
a. The supervisor asks you to state the different growth characteristics of pathogenic organisms on TCBS.
b. In what way does the TCBS agar inhibit growth?
Explanation / Answer
1.
a. We are looking for signs of Streptococcus activity on SBA plates.
The bacteria would cause lysis and complete digestion of red blood cells surrounding a colony.
And hence, the infection can be confirmed.
b. Because of the time and resources required to collect and process specimens, blood cultures should be obtained from children likely to have bacteremic pneumonia.
Since no specific question has been asked, only the first question has been answered
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