You are studying lactose metabolism and have been culturing E. coli in different

Question

You are studying lactose metabolism and have been culturing E. coliin different medias. One media has only glucose, one has only lactose, and a third has both glucose and lactose.

(1 pt)You notice that some of the cultures grown in the presence of both glucose and lactose are metabolizing lactose. Which type(s) of mutation(s) could cause this to occur?

(1 pt)You notice that some E. colicultures failed to grow in the presence of only lactose. You know that normal E. coliare able to metabolize lactose so something must be wrong. Which mutation(s) could explain this?

Explanation / Answer

I believe the correct options to be:

A) If you look at the lac operon mechanism, it can be seen that a mutation in lacl gene that is responsible for repressing the lac operon would result in its activation which will ultimately lead to use of lactose in any condition.

B) A mutation in CAP binding site in lac operon would result in no binding of polymerase so no matter what the condition is, proteins for lactose metabolim would not be produced so lactose would not be used.

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