You are studying a strain of bacteria that carries a temperature sensitive mutat

Question

You are studying a strain of bacteria that carries a temperature sensitive mutation in one of the genes required for DNA replication. The bacteria grow normally at the lower temperature, but when the temperature is raised they die. When you analyze the remains of the bacterial cells grown at the higher temperature you find evidence of partly replicated DNA. WHen the strands of this DNA are separated by heating, numerous single stranded DNA molecules around 1000 nucleotides long are found. Which of the proteins listed below are most likely to be impaired in these mutant bacteria?

Explanation / Answer

The protein named as the " Rho protein " likely to be impaired in these mutant bacteria. This particular Rho protein is highly inactive or nonfunctional at high temperature. Rho protein is important for bacteria in transcription termination, when this protein gets nonfunctional, the termination does not takes place during itrancription and numerous single stranded DNA molecules will be formed.

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