You are studying a strain of bacteria that carries a temperature-sensitive mutat

Question

You are studying a strain of bacteria that carries a temperature-sensitive mutation in one of the genes required for DNA replication. The bacteria grow normally at the lower temperature, but when the temperature is raised they die. When you analyze the remains of the bacterial cells grown at the higher temperature you find evidence of partly replicated DNA. When the strands of this DNA are separated by heating, numerous single-stranded DNA molecules around 1000 nucleotides long are found. Which of the proteins listed below are most likely to be impaired in these mutant bacteria?

Explanation / Answer

As already mentioned in the Q. that the when the DNA strands were separated by heating numerous single stranded DNA molecules of 1000nuclotides long are found which clearly indicating a positive response of DNA Polymerase, Helicase, Primase, Initiator of replication proteins H as well as single stranded binding proteins and thus manifesting its functionality

But, as mentioned there are 1000 nucleotides long stretches of DNA are found which demarcates the dysfunctional property of ligase enzyme which functions to seal the lagging strand short stretches of polymerized sequences and thus may prove to be responsible for incomplete replication.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.