You are studying a small polypeptide in solution and measure the linewidth (full

Question

You are studying a small polypeptide in solution and measure the linewidth (fullwidth-half-height) of one amide 1 HN signal as a function of sample pH:

pH LW (Hz)

1.5 2.33

2.0 0.95

2.5 0.52

3.0 0.38

3.5 0.34

4.0 0.33

4.5 0.34

5.0 0.38

5.5 0.52

6.0 0.95

6.5 2.33

7.0 6.68

7.5 20.45

8.0 63.98

Plot log(LW) vs pH, and provide an explanation for the observed behavior of the amide 1 HN signal assuming that R2 = 1/T2 = (LW) = R2,o + kex and that kex = kH+[H3O+ ] + kOH- [OH- ], estimate the 2nd order rate constants kH+ and kOH- from these data.

Explanation / Answer

Plot logLW (y axis) vs pH (x axis)

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