1.) A protease digest is done using trypsin. How many fragments would be produce

ID: 147096 • Letter: 1

Question

1.) A protease digest is done using trypsin. How many fragments would be produced?

21, 22, 42, 52

2.)Consider the most C-terminal fragment produced from a trypsin digest of human myoglobin. A titration curve of this fragment would produce:

How many inflection points with what approximate pKa values? Answer choices:

N-term (8.5), E-side chain(4.3), C-term (3.5): 3 inflection points

N-term (8.5), E-side chain(9): 2 inflection points

N-term (8.5), C-term (3.5): 2 inflection points

N-term (8.5), E-side chain(4.3 and 9), C-term (3.5): 4 inflection points

N-term (8.5), E-side chain(4.3), C-term (3.5): 3 inflection points

N-term (8.5), E-side chain(9): 2 inflection points

N-term (8.5), C-term (3.5): 2 inflection points

N-term (8.5), E-side chain(4.3 and 9), C-term (3.5): 4 inflection points

1.) A protease digest is done using trypsin. How many fragments would be produced?
Correct option:

2) inflection points with what approximate pKa values
Correct option:

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