DNA preparation 1. What is extracted from the cells for use in PCR? 2. What is t
ID: 135917 • Letter: D
Question
DNA preparation 1. What is extracted from the cells for use in PCR? 2. What is the purpose of using saline solution to rinse your mouthi? PCR Amplification 3. Write out the main steps of polymerase chain reaction (PCR) 4. Imagine that the DNA in the box below is your target DNA to be amplified. Highlight the two nucleotide sequences, outside your target sequence, where each primer should attach. Be mindful of the directionality of DNA 3'-AAATTCCGGTTAA CGCTTCCGGGAGAGGTAAA TTCCGGGAGAGGAT-5 5'-TTTAAGGCCAATT GCGAAGGCCCTCTCCATTT AAAGGCCCTCTCCTA-3'Explanation / Answer
1 In the process of PCR, DNA or Deoxyribonucleic acid extracted from the cell. the process comprises the separation of the cell membrane, proteins, and some material present within the cell.
2. The reason behind using warm saline solution for rinsing mouth as the antiseptic property of the saline solution acts to promote healing as well as relieves pain also. In most of the time, the dentist suggests rinsing mouth with this solution some days after surgery just to relieve pain and swelling.
3. The three main steps of the PCR :
Denaturation :
In this step, the double helical DNA strands denatured into a single strand by breaking the hydrogen bonds present into it This happens to the 94-95*C of temperature.
Annealing :
Annealing step involves the primers. These primers anneal to the DNA templates. This happens at 54-56*C.
Extending :
It is the third step of the PCR cycle.That is called the extension step. The other name of this step is the elongation step, It comprises the Taq polymerase attaches nucleotides to the primer which already been annealed. The temperature required to complete the process is 74-75*C
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