DNA extraction buffer contains detergent and salt, what are the purposes of dete
ID: 173145 • Letter: D
Question
DNA extraction buffer contains detergent and salt, what are the purposes of detergent and salt, respectively? What is the purpose of cold ethanol used in this lab? If you had extracted DNA from animal cells instead of plant cells, what cell barrier would have been different? What do plant cells have that animal cells do not? Do you think that DNA from animal cells would look the same as DNA from plant cells? Why or why not? Give an example that a scientist can do with extracted DNA. Explain why Mini-Prep method allow us to remove genomic bacterial DNA and to isolate plasmidsExplanation / Answer
4. DNA extraction buffer contains detergent and salt. The detergent is useful to dissolve the phospholipid bilayer of the cell membrane and other cell organelles. The salt is useful to dissolve the protein chains surrounding the DNA.
5. DNA is not soluble in ethanol. To make the DNA less soluble, ethanol is cooled or freezed. So, ethanol must be kept in ice.
6. Animal cells contain only cell membrane but not cellwall. The plant cells contain the cellwall made up of cellulose. The DNA extraction in plants can be acheived by adding cellulase enzymes to dissolve the cellulosic cellwall. Other secondary metabolites produced by plants, proteins and chloroplasts interfere with DNA extraction from plant cells. DNA extraction from aniimal cells require to withdraw blood sample. The major nonprotein compound interfering with DNA extraction from animal cells is heme.
7. The DNA sequences are same and universal in all types of cells but have different bases in the sequences. Generally, plant genomes are larger than animal genomes. The compounds like secondary metabolites and chloroplasts are not found in animal cells and these differences impact the DNA extraction methods also.
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