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1. This test is used to differentiate Neisseria species from one another: beta-h

ID: 133398 • Letter: 1

Question

1. This test is used to differentiate Neisseria species from one another:

beta-hemolysis

sugar fermentation

Gonocheck-II

d. latex agglutination

2. A lab technician plated a sample of bacterium on a specific medium. After incubation, she noticed the previously yellow medium was now a hot pink and there were many colonies now growing on the medium. What type of medium did she use?

A)

general purpose

B)

differential

C)

synthetic

D)

selective

a.

beta-hemolysis

b.

sugar fermentation

c.

Gonocheck-II

d. latex agglutination

2. A lab technician plated a sample of bacterium on a specific medium. After incubation, she noticed the previously yellow medium was now a hot pink and there were many colonies now growing on the medium. What type of medium did she use?

A)

general purpose

B)

differential

C)

synthetic

D)

selective

Explanation / Answer

1.

Ans : Most of the bacteria which produces acid by a fermentative pathway.  Neisseria spp. produce acid by an oxidative pathway. This is main difference because more acid is produced by fermentation than by oxidation. Some Neisseria species, e.g., Neisseria cinerea, produce acid from carbohydrates and suddenly overoxidize the acid to carbon dioxide.

Hence option (b) is correct.

2.

Ans: After incubation, she noticed the previously yellow medium was now a hot pink and there were many colonies now growing on the medium. What type of medium did she use.  Thange in color is that S. aureus ferments the mannitol, producing an acid, which, in turn, changes the yellow to pink.

This method is called as differential medium.

Hence option (b) is correct.