Experiment 11 prelab-Word ngs Review View ACROBAT Tell me what you want to .1|1N

Question

Experiment 11 prelab-Word ngs Review View ACROBAT Tell me what you want to .1|1Norm all!NoSpac_ Subtle Em... Emphasis Intens :E, 2: Heading 1 Heading 2 Title Subtitle Paragraph Styles B. You have a stock solution of sequencing primer that is 3.2 pmoYuL. How many ? of this sequencing primer will you place into your DNA sequencing reaction for a total of 6.4 pmol of sequencing primer? C. The total required volume for your sequencing sample is 12 ? L. What volume of sterile ddH2O is required in your sequencing sample?

Explanation / Answer

B)

3.2 pmol / ul means one ul stock solution contains 3.2 pmol sequencing primer.

so volume of sequencing primar in uL = 6.4 * 1.0 / 3.2 = 2.0 uL (answer).

C)

volume of the solution = 12 uL

as the volume change during the addition of sequencing primer is negligible.

so here volume of sterile H2O required = 12 uL

Related Questions

Navigate

• Browse (All)
• Browse E
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.