76 21 How might a better chromatographic separation of solutes be accomplished?

Question

76 21 How might a better chromatographic separation of solutes be accomplished? I3] What would happen if a pen was used to mark the starting line and ion application points? Be cause most pen ha ment in which ill disolvt in Chmteaphy lve in the chomo 141 Why is it necessary to keep the point of application small? How does the size of the applied sample area affect the chromatographic separation and the Re value? Because t eseects the Redention Valu(P Value pe the Re tention valx aluthe size aiso etlects inciessin g even inits the ke 1S) What ion(s) appear to remain in the stationary phase for Star [6] What ioms) resides in the mobile phase for the longest period of time?

Explanation / Answer

1) for better separation of solutes they should be taken in low concentration (less amount). The solutes will have better separation if a suitable solvent is used which has good affinity for one solute and less for other.

2) True. We should use pencils in place of pen. As pen uses ink (dye kind of material) which are organic compound and shows affinity (solubility ) in many organic solvents so may cause ambiguity in the recognization of peak of the actual compound to be isolated.

3) Correct

4) the ions which have more affinity for stationary phase will remain for longer period on the stationary phase.

If we have used cation exchange resin they will have negatively charged sites so have affinity for positive ions and the positive ions will remain longer period on the stationary phase

If we have used anion exchange resin they will have positively charged sites so have affinity for negative ions and the anions will remain longer on the stationary phase

5)

If we have used cation exchange resin they will have negatively charged sites so have affinity for positive ions and the negative ions will remain longer period on the mobile phase

If we have used anion exchange resin they will have positively charged sites so have affinity for negative ions and the postive ions will remain longer period on the mobile phase

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