Mark each statement below TRUE or FALSE and explain your choice. Credit given if

Question

Mark each statement below TRUE or FALSE and explain your choice. Credit given if your reasoning is correct. a) For a reaction, increasing [S] will make the reaction more energetically favorable and increase the reaction rate. b) Comparing the turnover rates of two enzymes at low [S] can be done by comparing the k_cat values of each. c) The coupling of ATP hydrolysis to a spontaneous reaction can increase the rate of the reaction d) Increasing [E] will not change the specificity constant for an enzyme-catalyzed reaction. The binding energy of two enzymes, E1 and E2 are -8 kJ mol^-1 and -12 kJ mol^-1 respectively. Based on this information, can you predict which enzyme will have a higher V_max? If yes, predict which enzyme has a higher V_max, and explain your answer. If no, explain why not.

Explanation / Answer

Yes we can determine which enzyme has the higher Vmax. E2 will have higher Vmax since the free energy released from the enzyme substrate complex will be higher that means E2 and its substrate will bind weakly and will be released as a product as a much faster rate than the E1 does. The E-S complex of the E2 will be converted to E+P at a faster rate than E1.

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