Mark all appropriate answer(s) You immunize a rabbit with DNP-BSA in order to ma

Question

Mark all appropriate answer(s)

You immunize a rabbit with DNP-BSA in order to make anti-DNP IgD. While giving a booster shot 3 weeks later you spill you DNP-BSA as the rabbit kicks you. The chemist who makes DNP conjugates is on vacation, but you do have some DNP-OVA. In order to induce a secondary immune response to DNP, you ONE BEST option is :

a. administer anti-DNP ab ( passive transfer of immunity) prior to challenge with DNP-OVA

b. boost with DNP-OVA every week.

c. immunize with OVA and wait 1&1/2 weeks before sensitization with DNP-OVA

d. start all over

e. use passive transfer of anti-OVA ab as you challenge with DNP-OVA

f. inject PBL (peripheral blood lymphocytes) from a syngeneic rabbit sensitized with OVA( that you have ready) (adoptive transfer of immunity) at the same time that you inject DNP-OVA

g. 1 week before injecting DNP-OVA, transfer CD8+ T cells from a syngenetic rabbit sensitized with OVA

h. none of the above, give up. Because of the carrier effect, you have to stick DNP-BSA

Thank you

Explanation / Answer

(not sure how you mark the ONE best answer as well as ALL appropriate answers...)

I like this question! The goal is to build immunity against DNP. Sounds like you can't inject DNP alone, but by hooking it to Bovine Serum Albumin, the rabbit will respond to it (and the BSA by accident). This duo is a hapten-carrier complex. I believe the body has to see both the hapten (DNP) and carrier (BSA) in boosters to work. (google books it)

In addition to that hint...

a) would work. We do it in my lab to trigger passive anaphylaxis. We grow cells to produce IgE-OVA, purify the IgE-OVA and then inject it to boost the immune response of a mouse challenged with OVA.

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