Determining the amino acid sequence of a protein (its primary structure) involve

Question

Determining the amino acid sequence of a protein (its primary structure) involves both chemical and enzymatic strategies in seven basic steps. A procedure for determining the N-terminal amino acid is:

reduction with 2-mercaptoethanol or dithiothreitol (DTT) followed by alkylation with iodoacetate specific hydrolysis with carboxypeptidase using an automated protocol derivatization with phenylisothiocyanate to form a PTH-derivative that is identified by chromatography specific hydrolysis with cyanogen bromide (CNBr) to yield a sulfonium ion that undergoes rapid intramolecular rearrangement to form the iminolactone

Explanation / Answer

Ans: derivatization with phenylisothiocyanate to form a PTH-derivative that is identified by chromatography

This method is called Edman degradation.

It is very common method  for determining the N-terminal amino acid.

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