A nonapeptide was determined to have the following amino acid composition9Arg)_2

Question

A nonapeptide was determined to have the following amino acid composition9Arg)_2(Gly)_2,(Phe)_2, His Lle, Met. 1-fluro-2-4-dinitrobenzene reacts with free amino groups in proteins to produce dinitrophenyl derivatives of amino acids. The native peptide was incubated with 1-fluoro-2,4-dinitrobenzene and then completely hydrolyzed;2,4- dinitrophenylhistidine was identified by HPLC. What does this tell you about the peptide sequence? When the native peptide was exposed to cyanogen bromide an octapetide and free glycine were recovered. What does this tell you about the peptide sequence? Incubation of the native peptide with trypsin gave a pentapeptide, a tripeptide, and free Arg. The peptides were separated and treated with FNDB,2,4-dinitrophenyl- histidine was recovered from the pentapeptide, and 2,4- dinitrophenylphenylalanine was recovered from the tripeptide. What does this tell you about the peptide sequence? Digestion with the enzyme pepsin produced was composed of z phe, and Gly. What does this tell you about the peptide sequence? What is the sequence of the nonapeptide?

Explanation / Answer

his - ile - phe - gly - arg - arg - phe - met - gly

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