PLEASE REFER TO DIAGRAM BELOW FOR I-J I) Write the protein sequence if there was

Question

PLEASE REFER TO DIAGRAM BELOW FOR I-J

I) Write the protein sequence if there was an insertion as shown below.

J) If a cell containing the mutation described in “i” were plated on a petri dish and incubated

overnight, what would you predict would be the phenotype of the colony compared to a wild type

colony? Why?

Bio 41 Summer 2017 Name: 1) Write the protein sequence if there was an insertion as shown below. 3 ACT-5 5, "TGA-3, 3' -AATGTGATCC GGCGCATACT TTAAATGGCA GCACTC GTAAATAATT TTGGCGATGT TGTCCACTCG-5 10 $1 10 21 31 41 51 J) If a cell containing the mutation described in "" were plated on a petri dish and incubated overnight, what would you predict would be the phenotype of the colony compared to a wild type colony? Why?

Explanation / Answer

5' CCGCGTATGAAATTTACCGTAGAACGTGAGCATTTATTAAAACCGCTACAACAGGTGAGC 3'

above sequence is without insertion and protein sequence is

PRMKFTVEREHLLKPLQQVS.

now if we insert the mentioned 3 nucleotide sequence then sequence will be-

5' CCGCGTATGAAATTTACCGTATGAGAACGTGAGCATTTATTAAAACCGCTACAACAGGTGAGC 3'

and protein sequence will be-

PRMKFTV#EREHLLKPLQQVS. that means protein sequence will be terminated in between and protein will not be formed.

2) we can not determine the phenotype of a colony. we do not have the information about what that protein does.

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