1. A patient reports to you with a skin condition where there is severe fracturi
ID: 97242 • Letter: 1
Question
1. A patient reports to you with a skin condition where there is severe fracturing of the skin all over the body. Tests show that blood biochemistry of the patient is normal for other factors. The patient appears to be exposed to a specific chemical at work place. You suspect that this chemical has an effect on the keratin intermediate filament network in the cells.
A.The chemical negatively interferes with the spontaneous assembly of keratin filaments
B. The chemical affects the expression of keratin proteins in the skin cells.
C. The chemical appears to interfere with the interaction of Keratin with Cadherins at the desmosomes
D. The chemical affects F-actin filament polymerization leading changes to cell shape.
E. The chemical increases phosphorylation of keratin subunits promoting disassembly
2. You have a culture of brewers yeast fermenting a brew of your signature craft beer. You wish to demonstrate the process of Mitochondrial protein translocation to a friend using this brewers in the culture. You express a mitochondrial matrix protein tagged with GFP. What will your friend observe in the yeast cell?
A. The mitochondrial protein fluoresces Green labeling the mitochondria.
B. The mitochondrial protein fluoresces in the endoplasmic reticulum of the cell.
C. The mitochondrial protein fluoresces Green in the entire cytoplasm.
D. There is no fluorescence signal in the cell.
A. The mitochondrial protein fluoresces Green labeling the mitochondria.
B. The mitochondrial protein fluoresces in the endoplasmic reticulum of the cell.
C. The mitochondrial protein fluoresces Green in the entire cytoplasm.
D. There is no fluorescence signal in the cell.
Explanation / Answer
Correct answer is:D. The chemical affects F-actin filament polymerization leading changes to cell shape.
Correct answer is:D. There is no fluorescence signal in the cell.
Reason:Since the protein will not be able to enter the TIM complex due to requirement of ATP and proton gradient,no fluorescence will occur.
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