1.What is the charge on the molecule at the isoelectric point? 2. (2) At the iso

Question

1.What is the charge on the molecule at the isoelectric point?

2. (2) At the isoelectric point, is each of the sites (labeled A, B, and C) protonated or unprotonated?

Site A: Pronated or unpronated?

Site B: Pronated or unpronated?

Site C:Pronated or unpronated?

The triprotic form of the amino acid glutamic acid is shown below, along with the pKa value for each ionizable site. B 9.7 A 2.2 H2 H2 C 4.3 OH For this molecule, pI-3.3 For this molecule, pI -3.3. (1) What is the charge on the molecule at the isoelectric point? (2) At the isoelectric point, is each of the sites (labeled A, B, and C) protonated or unprotonated? site A:

Explanation / Answer

1) pI = 3.3 means it is a acid medium so the glutamic molecule can has a positive (+Ve) charge.

2) If the pka of each one is higher for each side will be protonated and viceversa.

Site A: Unprotonated
Site B: Protonated
Site C: Protonated

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