calculate the resolving power of the TOF-MS using the 10% valley formula and the

Question

calculate the resolving power of the TOF-MS using the 10% valley formula and the half-width formula. NOTE THE ANSWERS SHOWN ARE INNCORRECT. Please provide correct methods and steps for each formula.

e eluate from a high-performance liquid chromatography (HPLC) separation was analyzed using electrospray time-of-flight mass spectrometry (TOF-MS). The molecular ions of two peptides, each with a mass of 597.0 Da, areseparated with a 10% valley between them. The molecular ion peaks have a width at half-height of 0.0243 Da and differ in mass by 0.0510 Da. Calculate the resolving power of the TOF-MS using the 10% valley formula and the half-width formula. Resolving power by the 10% valley formula Resolving power by the half-width formula Number Number 1170.59 21814.81 Previous Give Up & View Solution ) Check Answer 0 Next Ex Hint For the two methods, the denominator is defined in different ways. In one method the denominator is the separation of the two peaks when the overlap of their bases is 10% of the maximum peak height in the other method, the peak width at half the maximum height is used.

Explanation / Answer

according to 10% valley method

resolving power = m / dm

where

dm = difference in masses

given

m = 597

dm = 0.0510

so

resolving power = 597 / 0.0510

resolving power = 11705

so

the resolving power by the 10% valley formula is 11705

now

we know that

according to half width method

resolving power = m / dm

where

dm = half height value

given

m = 597

dm = 0.0243

so

resolving power = 597 / 0.0243

resolving power = 24567.9

so

the resolving power by the half width formula is 24567.9

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.