1.Using the absorbance of the spinach extract and the equation of the trendline,

Question

1.Using the absorbance of the spinach extract and the equation of the trendline, determine the concentration of the extract solution. Report the concentration in moles/L (M).

2. Calculate the number of rams of chlorophyll-a in the 25ml, spinach solution.

3. Calculate the concentration of the chloropyhll-a soultion in spinach (mg chlorophyll-a/g spinach)

Trendline: y=1609x + .0055

Absorbance spinach extract lamda max: .329

Absorbacne spinach extract, 750 nm: .023

Corrected absorbance: .306

Mass of Spinach: .1876g Total Volume of spinach: 25mL

Explanation / Answer

We have,

Absorbance = 0.306

y = 1609x + 0.0055

0.306 = 1609x + 0.0055

1. concentration of extract solution for chlorophyll-a (x) = 0.0002 M

2. Grams of chlorophyll-a in extract solution = 0.0002 mol/L x 893.51 g/mol x 0.025 L = 0.0045 g

3. Concentration of chlorophylll-a in spinach solution = 4.5 mg/0.1876 g = 24mg/g

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