A-E lists experiments performed on an unknown carbohydrate. For A-E, BRIEFLY ide

Question

A-E lists experiments performed on an unknown carbohydrate. For A-E, BRIEFLY identify the specific information the experiment provides about the identity and/or structure of the unknown.

A. Complete hydrolysis of the carbohydrate gave glucose and fructose in a 1:1 ratio.

B. Addition of -glycosidase to the carbohydrate results in formation of glucose and fructose in a 1:1 ratio. Addition of ß-glycosidase gives the same exact results.

C. Reaction of the carbohydrate with Cu2+ does not result in product formation.

D. CH3I treatment of the carbohydrate followed by complete acid hydrolysis provided 2 products. One of the products is 1, 3, 4, 6, tetra-O-methyl-fructofuranose

E. MS analysis provided a molecular weight of 342.3 g/mol

F. Draw the structure of the carbohydrate below.

Explanation / Answer

The carbohydrate discussed above is sucrose and a common table sugar that is having a molecular weight of 342.3 g/mol. The complete hydrolysis of the sucrose gives glucose and fructose in 1:1 ratio. when hydrolysed by both enzymes alpha-glucosidase and beta-glucosidase both gives an equimolar mixture of glucose and fructose. The fehlings test gives negative for the sucrose and when treated with methyl iodide the sucrose gives 1,3,4,6-tetra-O-methyl-D-fructose as one of the product. The mass spectrometric analysis which is generally performed for determining the molecular weight gives 342.3 g/mol. The structure of sucrose is given everywhere. Please check.

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