MICROBIOLOGY ONLINECULTIVATION OF BACTERIA - STUDY HELP The information in this
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Question
MICROBIOLOGY ONLINECULTIVATION OF BACTERIA - STUDY HELP
The information in this document is helpful in preparing for the Pre-Lab Quiz on Canvas and the Cultivation of Bacteria assignment quiz on Late Nite Labs.
Instructions:
Go to your Late Nite Labs course site and click on the Cultivation of Bacteria link. The lab manual content will appear on the right side of the screen.
PRINT and READ all of the information found in the Cultivation of Bacteria lab manual.
Answer the questions on this document.
Questions
True or False: Disease-causing bacteria can become resistant to disinfection methods.
True or False: Disease-causing bacteria can only be found in hospitals, nursing homes, and on the skin of people who have wounds.
List the physical characteristics used to distinguish between different types of bacteria.
List the factors that determine bacterial colony shape.
List the ways of differentiating between bacterial and fungal growth (include shape and size).
List three media in which bacteria can grow.
What are the advantages of using agar slants?
True or False: In the streak plate method, the bacterial density increases with each streak.
True or False: The quadrant streak method is commonly used to isolate high-density cultures.
True or False: Bacterial isolation allows a scientist to establish which individual species isresponsible for the results of an experiment.
Suppose you use the streak plate method to culture bacteria. Label each streak in the picture below.
Which streak plate would have the lowest cell density?
True or False: In the streak plate method of isolation experiment, you will use a wire rod to streak bacteria.
True or False: In the streak plate method, failure to sterilize the rod after the first streak may result in a similar concentration of bacteria in the second streak.
The following diagram is an example of the spread plate method of culturing bacteria.
A test tube contains a bacterial culture that is very dense (like “A” in the above diagram). Are you likely to see individual colonies on the corresponding spread plate?
AFTER YOU HAVE ANSWERED ALL OF THE QUESTIONS, TAKE THECULTIVATION OF BACTERIA (COB) PRE-LAB QUIZ ON CANVAS.
Then, go to the Cultivation of Bacteria Lab on Late Nite Labs and complete ALL lab activities.
After you have completed ALL Cultivation of Bacteria lab activities, you can take your Late NiteLabs assignment quiz.
You will need to know all of the information on this study help document for your PRE-LABQUIZ on CANVAS and for your LATE NITE LABS assignment quiz on the Late Nite Labs site.
Be sure and read the quiz questions carefully. Some questions might address the STREAKmethod and others the SPREAD method. Keep them straight.
Explanation / Answer
1. The answer is false. Disease causing bacteria are not resistant to disinfection methods.
2. The answer is True. More disease causing or infectious bacteria are found in hospital, nursing homes and diseased people. But the infectious organisms are also found in polluted environment also.
3. The first and the prior method to distinguish the bacteria is gram staining technique, by this method we can identify the bacteria either gram positive or gram negative. Gram positive bacteria have more peptidoglycan in cell wall than gram negative bacteria. Acid-fast staining is used to identify the mycobacterium sps., and endospore staining, flagellar staining, negative staining are used to detect the spore formers, motility of the bacteria and presence of capsule or not respectively.
4. A bacterium grows on agar media and form different colonies. Different species of bacteria can produce different colonies.
Colony shape varies round to irregular to filamentous and rhizoid (root-like)
Colony size can vary from large to tiny colonies
Opacity of colony: Transparent, opaque, translucent, iridescent
Elevation of colony on the agar
Colony surface on agar plate - Smooth, glistening, rough, dull, wrinkled
Colony texture: Butyrous, viscid, brittle/friable and mucoid
5. Bacteria are prokaryotes and unicellular organisms, mostly the bacteria are multiply by asexual reproduction - binary fission. Fungi are eukaryotic organisms and they grow slowly than bacteria. fugi resproduce by asexual reproduction as well as sexual reproduction also.
6. List of the media for bacterial growth are plate count agar, Nutrient agar and Trypticase soy agar
7. Agar slants are used for preservation of cultures and also useful for the transporting of cultures
8. The answer is False. In streak plate technique, in each streak the bacterial density decreases
9. The answer is True.
10. The answer is True
11. The 1st streak has the greatest bacterial density
12. The 4th streak have the lowest cell density
13. The answer is True. The wired rod is the inoculation loop is used to streak
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