You are faced with separating two different proteins with identical mass. Protei

Question

You are faced with separating two different proteins with identical mass. Protein A is a rod-like molecule with a great deal of secondary srtucture and protein B is a globular protein that is roughly spherical. What methods could be used to separate these two proteins? (check all that apply).

Ion-exchange chromatography

Two-dimensional electrophoresis

SDS PAGE

Size exclusion chromatography

Affinity chromatography

Ion-exchange chromatography

Two-dimensional electrophoresis

SDS PAGE

Size exclusion chromatography

Affinity chromatography

Explanation / Answer

Ion-exchange chromatography ,

Two-dimensional electrophoresis

Affinity chromatography

by using these three methods we can separate.

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