The data to the right was collected for a certain enzyme-catalyzed reaction. Use

Question

The data to the right was collected for a certain enzyme-catalyzed reaction. Use these data to determine the values of Rmax (the max rate of the enzyme, often called the max velocity, Vmax) and Km (the Michaelis-Menten constant of the enzyme). Answer the three parts below.

Map ddd University Science Books presented by Sapling Learning onald McQuarrie Peter A Rock.Ethan Gallogly The data to the right was collected for a certain enzyme-catalyzed reaction. Use these data to determine the values of Rmax (the The data to the right was collected for a certain enzymeazd SI mlLuol- Lmin edS(umo L R(umol L1 min maximum rate of the enzyme, often called the maximum velocity, Vmax) and KM (the Michaelis-Menten constant of the enzyme). Answer the three parts below 3.0 5.0 1.0 x101 30.0 10.4 14.5 22.5 33.8 40.5 a) If you were to plot this data to graphically determine Rmax and Kw 300 using a Lineweaver-Burk plot, what would you plot? If you would 90.0 use the original data, enter the original value in the corresponding blank IS] (umol L- Graph as R (umol L1 min) Graph as Number Number 3.0 10.4 Number Number 1.0 x 101-011 1.0× 101 11 22.5 b) Which of these graphs would you use to graphically determine Rmax and KM? co l O Previous 0'a ve up & View Scilullon 0 Check Answer 0 NextExit Hint

Explanation / Answer

a) To plot we would be using the data as,

[S] umol.L-1          R (umol.L-1.min-1)

3 ----> 0.333            10.4 ----> 0.096

1x10^1 -> 0.1          22.5 -----> 0.044

b) We would use,

graph B

to calculate Vmax and Km

c) Rmax = 1/y-intercept = 1/0.02241 = 44.62 umol.L-1.min-1

Km = 1/slope = 1/0.22399 = 4.46 umol.L-1

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