The data to the right were collected for the bovine carbonic anhydrase-catalyzed

Question

The data to the right were collected for the bovine carbonic anhydrase-catalyzed reaction described by co2(aq)+h2o(l) right arrow h+(aq)+hco -3(aq) Use these values to determine the value of Km, the Michaelis-Menten constant. Answer the four parts below. If you were to plot this data to graphically determine Rmax and Km using a Lineweaver-Burk plot, what would you plot for the following data? If you would plot the original data, enter the original value in the corresponding blank. Calculate the slope using the data points from part (a). Then determine the y-intercept using the second point. Calculate the slope using the data points from part (a). Then determine the y-intercept using the second Use the y-intercept to calculate Rmax Use Rmax and the slope to calculate Km.

Explanation / Answer

a) We will plot a graph between 1/[CO2] v/s 1/[R]

So will plot "x" as 1/[CO2]

(i) 2.50 ---> graph as 0.4 L /mmole

(ii) 5---> graph as 0.2 L /mmole

at "y" axis we will plot R as 1/[R]

(i) 48.61 as 0.0206 L s (micromole)-1 = 20.572 L s (mmole)-1

(ii) 80.28 as 0.0125 L s (micromole)-1 =12.456 L s (mmole)-1

b) slope will be:

y2-y1/x2-x1 = 40.577

y = mx + b

b = y-mx

Where b= intercept

m =slope

b = 20.572 -40.577(0.4) = 4.34

c) Rmax = 1/ intercept = 1/4.34 = 0.2304 mmole / L s

The calculations are made in excel as:

micromol / L.s L.s/micromole L.s/mmole slope intecept [CO2] 1/[CO2] [R] 1/[R] 1/[R] x2-x1 y2-y1 y2-y1/x2-x1 4.340906 x1 2.5 0.4 y1 48.61 0.020571899 20.57189879 -0.2 -8.1154962 40.57748098 x2 5 0.2 y2 80.28 0.012456403 12.45640259 Rmax 0.230367

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