The data to the right was collected for a certain enzyme-catalyzed reaction. Use

Question

The data to the right was collected for a certain enzyme-catalyzed reaction. Use these data to determine the values of R_max (the maximum rate of the enzyme, often called the maximum velocity, V_max) and K_M (the Michaelis-Menten constant of the enzyme). Answer the three parts below. If you were to plot this data to graphically determine R_max and K_M using a Lineweaver-Burk plot, what would you plot? If you would use the original data, enter the original value in the corresponding blank. Which of these graphs would you use to graphically determine R_max and K_M?

Explanation / Answer

Graph will be between 1/[S] and 1/[R]

[S] ---> 5 = 2

            20 = 5

R ----> 22 = 4.5

            65 = 1.54

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