Describe the probable effects on gene expression in the lac operon of a mutation

Question

Describe the probable effects on gene expression in the lac operon of a mutation in (a) lac operator that deletes most of O1; (b) the lacl gene that inactiveates the repressor; (c) the promoter that alters the region around position-10 Describe the probable effects on gene expression in the lac operon of a mutation in (a) lac operator that deletes most of O1; (b) the lacl gene that inactiveates the repressor; (c) the promoter that alters the region around position-10 (b) the lacl gene that inactiveates the repressor; (c) the promoter that alters the region around position-10

Explanation / Answer

Ans a: The mutation in lac operator prevents the repressor protein from binding to the operator causing the constitutive expression of lac genes even in absence of lactose.

Ans b:the mutaion in lac I gene does not produce repressor protein and constitutively expresses the genes for lactose metabolism even in absence of lactose.

Ans c: the mutation in promotor prevents RNA polymerase from binding to the promotor and halting the expression of lactose metabolising proteins even in presence of lactose.

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