To determine the amount of iron (Fe3+) within the stem and floret parts of brocc

Question

To determine the amount of iron (Fe3+) within the stem and floret parts of broccoli and examine if there is a difference in the concentration of iron available in those parts by conducting a spectrophotometric analysis on the vegetable by combining the iron with potassium thiocyanate (KSCN).

We are investigating the concentrations of iron content within different parts of broccoli by comparing it to a calibration plot created from five different standards.

The following chemical reaction took place during this experiment:

Standard solutions in this experiment contained the following amounts of iron (III) chloride (FeCl3) and hydrochloric acid (HCL):

Spectrophotometric analysis was conducted for the five standard solutions above at a maximum wavelength of 463.6 nm and the following results were found:

Explanation / Answer

1. Determine the equation of the line by linear regression using the data obtained from the five standard solutions. The equation of the line is y = 9192.469x + 8.6125E-3

2. I could not answer the next questions because the absorbance od the diluted aliquot is not given. all subsequent calculations will start from there.

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