2 plates received 10 microliters from the same dilution tube. the first plate ha

Question

2 plates received 10 microliters from the same dilution tube. the first plate had 293 colonies, whereas the second had 158 colonies. a Suggest reasonable sources of error. 2 plates received 10 microliters from the same dilution tube. the first plate had 293 colonies, whereas the second had 158 colonies. a Suggest reasonable sources of error. 2 plates received 10 microliters from the same dilution tube. the first plate had 293 colonies, whereas the second had 158 colonies. a Suggest reasonable sources of error.

Explanation / Answer

The dilution is still too concentrated and colonies formed from more than one bacterium. If we decrease the concentration to one-tenth of the initial concentration,then we will get 20-30 colonies on each plate.

293/10 = 29.3 colonies and 158/10 = 15.8 colonies, this is roughly 20 - 30 colonies on each plate.

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