The map of the lac operon is: POZY The promoter (P) region is the start site of
ID: 886070 • Letter: T
Question
The map of the lac operon is: POZY
The promoter (P) region is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutationally altered promoters (P-) cannot bind RNA polymerase. Certain predictions can be made about the effect of P- mutations. Use your predictions and your knowledge of the lactose system to complete the following table. Insert a “+” where an enzyme is produced and a “-“ where no enzyme is produced. The first one has been done as an example.
Beta-galactosidase
Permease
No lactose
Lactose
No lactose
Lactose
I+P+O+Z+Y+/I+P+O+Z+Y+
-
+
-
+
I+P+O+Z-Y+/I+P-OCZ+Y+
I+P+O+Z+Y+/I+P+OCZ+Y-
I-P-O+Z+Y+/I-P+OCZ-Y+
I+P+O+Z+Y+/I+P+O+Z+Y+
I-P+OCZ+Y-/I+P+O+Z-Y+
I-P-O+Z+Y+/I+P+O+Z-Y+
ISP+O+Z+Y-/I+P+O+Z-Y+
I+P+O+Z+Y-/I+P+O+Z+Y-
Beta-galactosidase
Permease
No lactose
Lactose
No lactose
Lactose
I+P+O+Z+Y+/I+P+O+Z+Y+
-
+
-
+
I+P+O+Z-Y+/I+P-OCZ+Y+
I+P+O+Z+Y+/I+P+OCZ+Y-
I-P-O+Z+Y+/I-P+OCZ-Y+
I+P+O+Z+Y+/I+P+O+Z+Y+
I-P+OCZ+Y-/I+P+O+Z-Y+
I-P-O+Z+Y+/I+P+O+Z-Y+
ISP+O+Z+Y-/I+P+O+Z-Y+
I+P+O+Z+Y-/I+P+O+Z+Y-
Explanation / Answer
No lactose Lactose No Lactose Lactose
I+P+O+Z-Y+/I+P-OCZ+Y+ - + - +
I+P+O+Z+Y+/I+P+OCZ+Y- - + + -
I-P-O+Z+Y+/I-P+OCZ-Y+ + + - -
I+P+O+Z+Y+/I+P+O+Z+Y+ - + - +
I-P+OCZ+Y-/I+P+O+Z-Y+ + + - -
I-P-O+Z+Y+/I+P+O+Z-Y+ + + - -
ISP+O+Z+Y-/I+P+O+Z-Y+ - - - +
I+P+O+Z+Y-/I+P+O+Z+Y- - + + +
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