You have purified total double-stranded DNA from Varicella Zoster. The purified

Question

You have purified total double-stranded DNA from Varicella Zoster. The purified DNA was dissolved in TE (Tris-EDTA) buffer to make a total volume of 100 microliters to form the "Original DNA Sample" You need to determine the concentration (micrograms/microliters) of this DNA sample. To do this, you took 2 micro Liters from the "Original DNA sample" and added these to 998 microliters of TE in a spec. cuvette , making a total volume of 1000 microliters or 1ml. Next, you used a spectrophotometer to determine the absprbance of 260nm of light (A 260) for this 1 ml of DNA-TE solution in the cuvette and the A260=0.095

1) What is the concentration (micrograms/microliters) of the DNA soloution in the in the cuvette?

2) What is the concentration (microgram/microliteres) of the DNA in the original DNA sample?

3) How many microliters of the original DNA sampe would you need to use in an experiment that required a total of 15micrograms DNA from this viral DNA sample?

***I know that double stranded DNA: A260 of 1.0=50 micrograms/mlWould it be correct the for #1 to do 0.095*50= 4.75micrograms/ml? the questions however asks for untis of micrograms/microliters, so does this change, if so how? and then I don't know how to find the other parts of the question. The only example we were given was A260 was determined to be 0.1 determine dsDNA concentration. and we did .1*50=5 Micrograms/ml

Explanation / Answer

Answer:

1) What is the concentration (micrograms/microliters) of the DNA soloution in the in the cuvette?

2) What is the concentration (microgram/microliteres) of the DNA in the original DNA sample?

3) How many microliters of the original DNA sampe would you need to use in an experiment that required a total of 15micrograms DNA from this viral DNA sample?

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