You have previously identified the gene LIV-1OO, that is expressed in liver cell

Question

You have previously identified the gene LIV-1OO, that is expressed in liver cells. Your research advisor asks you to check whether LIV-1OO is expressed in any other tissues. You isolate mRNA from brain, heart, skin and muscle cells and run the mRNA on a gel along with a sample from liver; after you finish electrophoresis you transfer (''blot") the mRNA to a piece of filter paper (this is called a Northern blot). You then make a UV-100 probe that consists of the antisense sequence of the last exon of LIV-WO (see below, Figure 1) and allow it to hybridize to the mRNA on the filter. The probe is radioactively labeled so if you expose the filter to film the radioactivity will expose the film and you can see where your probe has hybridized. Much to your surprise, you obtain the results shown in Figure 2. In what tissues is LIV-1OO expressed? How do you interpret the different sized bands seen in the None blot?

Explanation / Answer

A. LIV-100 is expressed in all the tested tissues except brain.

B. The different sized bands are possibly due to alternative splicing of the LIV-100 pre-mRNA.

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