You have prepared two culture tubes (tube A and lube B) containing thioglycolate

Question

You have prepared two culture tubes (tube A and lube B) containing thioglycolate medium (FTM), which allows oxygen to diffuse only in the upper part of the medium. You inoculate two tubes a and B with one of two different organisms. After incubation for an appropriate length of time at the optimal growth temperature of each bacterium, you examine the cultures and observe the following. The bacterium in tube A grew only at and near the surface of the culture medium the bacterium in tube B grew evenly throughout the entire culture medium Define the two organisms in terms of their relationship with oxygen. Explain why the two organisms grew according to the observed pattern.

Explanation / Answer

Strictly aerobic bacteria requiring oxygen for growth like Pseudomonas spp are most likely to grow toward the surface of the broth.

Strictly anaerobic bacteria, which are incapable to grow in the presence of oxyygen, grow at the bottom of the broth.

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