One method of DNA sequencing use dideoxynucleotide their incorporation of a to s

Question

One method of DNA sequencing use dideoxynucleotide their incorporation of a to stops further DNA synthesis because a) the back a 3'-hydroxyl group, nucleotide cannot attach to it. b) the -lack 5'-phosphate group cannot attach to it. c) the cannot bind of the RNA primer d) the did cleaves the DNA polymer necessary for DNA synthesis Where does translation occur for protein desired to be imbedded in membrane or excreted from the cell? a) in the free cytoplasm b) in plasma membrane c) endoplamic d) smooth end plasmic Which of the following process is an exception to the central and typically occur in viruses? a) DNA makes RNA b) RNA makes DNA c) RNA makes polypeptide d) polypeptide make RNA During of a viral genome from host chromosome, the virus accidentally takes a piece of host DNA with it. This may occur a) while the virus is converting from lytic to stage b) resulting in generalized transduction c) at any time d) while the virus is converting from lysogenic to lytic stage. During of a viral genome from host chromosome, the virus accidentally takes a piece of host DNA with it. This may occurs (a) white the virus is converting from lytic to lysogenic stage (b) resulting in generalized transduction (c) at any time (d) white the virus is converting from lysogenic to lytic stage. Renaturation on curves of DNA isolated from organisms have shown 3 different categories of DNA. All of the following are correct paring of different DNA type except? (a) highly repetitive DNA - so DNA around centromere (b) middle repetitive DNA - (c) semi repetitive - telomere sequence (d) dry blood clothing factor Exceptor viruses the genetic sequence read during translation is non overlapping which means (a) each codon can only code for one specific amino acid, not different amino acid (b) there is only one codon for each amino acid (c) each codon for same amino acid in all reading frames (d) once the initiation codon is read, any single within the mRNA is read as part of only one codon. Incase of by effect where the absence of the H substance makes the expression of the ABO gene. This illustrate a classic example of? (a) epistasis (b) modernal effect (c) variable expressivity (d) phenocopy

Explanation / Answer

Dideoxy nucleotides (ddNTPs) are used in Sangers method for DNA sequencing. They inhibit chain elongating action of DNA polymerase. ddNTP lack 3'-OH group. Because of the absence of 3'-OH group, condensation reaction which occurs between 5' phosphate of the added nucleotide and 3'-OH group of the previous nucleotide will not occur and phospodiester bonds cannot be created. So, chain elongation and further DNA synthesis stops. Therefore, the answer for this question is option (a) the dideoxynucleotide lack 3'-OH group, so other nucleotide cannot attach to it.

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